Assalamualaikum w.b.t
These are the format regards our microbiology practical exam that have been confirm with Dr.Shams..quite long but try to read it throughly to ease ur revision..
There'll be 10 spots :
(i) 3 spots - slides of microorganisms
(ii)7 spots (mixed) either : - empty media & culture media
-serological techniques: ( ELISA , complement fixation test(CFT),single radial immunodiffusion ONLY)
-antibiotic sensitivity test
*For the first 5 spots,there are 2 questions to be ask :
1) Identification of the spots/substance
2)Question based on the identification (1 question only)
*For the last 5 spots,there only one question ONLY.
1) Identification of the spots/substance
Duration : 1 and 1/2 minutes for each spots
*The questions are general & short direct questions
*The questions that'll be ask more or less like in the given revision paper (but try not too dependent on it)
Revision Paper
-please cancel out the cooked meat medium --> it will not be ask
-please cancel the question on ELISA regards the comment either the test is (+ve/-ve) ---> it also not to be ask
ELISA
-there'll be questions to complete out the diagram or drawing pictures regards of it methods for Ag-Ab reaction
Antibiotic sensitivity Test(the questions probably on):
-Definition of MIC & MBC
-Identification of tube of having the MIC
-Clinical significant of MIC & MBC
N/B : Dr.Shams said that there's NO need to know about the procedures of each test unless it is mentioned in the revision paper..
Any mistake from me,i'm apologizing for it..and i'm really sorry for the late annoucement as it's hard to get a confirmation regards about the format..thank u..
GUDLUCK & BEST WISHES~
bitaufiq wannajah~
Dante Dante
microb pract exam:
we learned 8 slides but only 3 of them will come up in the exam, so i've been told:
1. Staph by gram stain.(violet color in clusters)
2. Strep by gram stain.(violet color in chains)
3.pneumococci/lanceolated diplococci by gram stain,(violet color in diplococci)
4.diptheriods by gram stain(violet color bacilli)
5.Acid fast bacilli by Ziehl Nelsen stain(pink rods against blue pus cells).
6. Neisseria by gram stain(RED color bcoz the only one which is gram -ve)
7.Neisseria by methylene blue stain(blue color in blue pus cells).
8. Neisseria from culture(the only slide which shows PINK diplococci!)
diphteroid
acid fast bacilli Z.N stain
G +ve lanceolated diplococci pneumococci
G +ve staphylococci-grape like cluster
gram +ve streptococci (Arrange in chain)
N.meningitidis (culture)
N.meningitidis gram stain (smear)
N.meningitidis methylene blue
alpaha hemolytic strept
bacitracin test
beta hemolytic strept
staph on blood
beta staph on blood
heat resistant
mcconky's agar
complement fixation test
disc diffusion
ELISA plate
sugar fermentation with acid n gas
sugar fermented-acid only
no sugar fermentation
Novobiocin
nutrient broth
optochin
phage typing
selenite broth
single radial immunodiffusion
staph on MSA
staph on nutrient
TB
TCBS
tube diution test
peptone
Thats All!
Original Source:
Thanks to them for uploading this photos to alexmedicalteam.
